Mycobacterium smegmatis|364246 |BNCC

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Mycobacterium smegmatis-BNCC
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  • Mycobacterium smegmatis-BNCC

Mycobacterium smegmatis

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  • number：364246
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    The colony has a diameter of 1-2mm, irregular shape, serrated edges, opaque, white on the front and light yellow on the back, rough surface, dry texture, no pigment production, G+(blue purple), Bacillus, purity: pure
Standard strain Quantitative strain DNA extraction
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Mycobacterium smegmatis
Subculture procedure ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions 37 ℃; 3-5 days; aerobic;
Storage conditions 2-8 ℃
Safety level 2
morphology The colony has a diameter of 1-2mm, irregular shape, serrated edges, opaque, white on the front and light yellow on the back, rough surface, dry texture, no pigment production, G+(blue purple), Bacillus, purity: pure
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