BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. During the cultivation process, the agar plate needs to be kept moist, and 7H10 broth can be added. |
| Growth conditions | 37 ℃; 2-3 weeks; 5%CO2; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped |
| Sharing mode | Public welfare sharing |