Birmingham Legionella|364378 |BNCC

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Legionella birminghamensis

Culture medium	BNCC360928
Description	Buffered Charcoal Yeast Extract Agar
Composition	Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C₅H₅KO₅):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe₄(P₂O₇)₃):0.25g
Growth conditions	37 ℃; 24-48h；5%CO2；
Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, irregular shape, irregular edges, opaque, milky white on the front, raised in the middle, smooth surface, moist texture, easy to pick up, G - (red), Bacillus subtilis
Sharing mode	Public welfare sharing