BeNa Culture Collection
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| Subculture procedure | Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 30 ℃; 24-48 hours; anaerobic; |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony has a diameter of 2-4mm, irregular shape, irregular edges, white front, raised middle, smooth surface, bright surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |