Ulcerative mycobacteria|368043 |BNCC

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Mycobacterium ulcerans MacCallum et al.

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	30 ℃; From February to June; aerobic; (Transfer and culture the bacterial solution after rejuvenation for 2 months)
Subculture procedure	① Prepare 1 bottle of liquid culture medium, about 200mL; ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid culture medium; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The liquid culture medium is turbid, the agar layer is obvious, the acid fast staining is positive, the bacteria are pure, and the purity is pure
Sharing mode	Public welfare sharing