Salmonella enterica serovar Typhimurium|185946 |BNCC

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Salmonella enterica subsp.

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	37°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	2
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grayish-white Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

Salmonella enterica subsp.

Storage conditions: 2~8 ℃

No. 185946

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, aerobic, nutrient gravy agar, 18-24h. Nutrient gravy agar: peptone 5.0g, beef extract 3.0g, NaCl 5.0g, agar 15.0g (not included in liquid medium), distilled water 1.0L, pH 7.0. [Note] Adding 5mg MnSO4·H2O when culturing Bacillus is beneficial to produce spores.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 18-24h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 1-2mm; Shape: round ; Edge: neat ; Transparency: opaque color: off-white ; Protuberance: middle bulge ; Surface: bright and smooth ; Texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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