BeNa Culture Collection
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| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 28 ℃; 3-4 days; aerobic; |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular with irregular margins; opaque; upper surface white; later produces cyan-blue spores; centrally convex; surface rough and wrinkled; texture dry and firm; G+ (blue-violet); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |