Pine Firmicutes|370713 |BNCC

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Pine Firmicutes-BNCC
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  • Pachyramichloridium pini-BNCC

Pachyramichloridium pini

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  • number：370713
  • Form：
    Filamentous fungi, migratory growth, mycelium with gray green on the front and black on the back
Standard strain Quantitative strain DNA extraction
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Pachyramichloridium pini
Subculture procedure ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions 18 ℃; 10-15 days; aerobic;
Storage conditions -80 ℃
Safety level 1
morphology Filamentous fungi, migratory growth, mycelium with gray green on the front and black on the back
Sharing mode Public welfare sharing
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