Active Mediterranean bacteria|372807 |BNCC

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Mediterraneibacter gnavus

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic
Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate is about 200 μ L). Place the plates in an anaerobic environment for 24 hours to equilibrate. ④ Apply evenly and transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; Gram-positive (blue-violet); cocci; purity: pure
Sharing mode	Public welfare sharing