BeNa Culture Collection
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| Culture medium | BNCC360812 |
| Description | Concentrated sugar Chatelier agar |
| Composition | Sucrose:200.0g, NaNO3:3.0g, MgSO4.7H2O:0.5g, KCl:0.5g, FeSO4.4H2O:0.01g, K2HPO4:1.0g, Agar:15.0g, pH:6.0-6.5(25℃) |
| Growth conditions | 24 ℃; 7-10 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 fresh tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony has a diameter of 0.5-1mm, irregular shape, irregular edges, opaque, gray white front, raised middle, rough surface, dry texture, G+(blue purple), square shape |
| Sharing mode | Public welfare sharing |
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