Trichoderma reesei|191533 |BNCC

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Trichoderma reesei

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi. Colonies are clearly visible on comprehensive PDA medium. Initial mycelium is white, dense, and vigorous, later producing yellow spores.
Sharing mode	Public welfare sharing

Trichoderma reesei

Storage conditions: 2~8 ℃

No. 191533

Product format: freeze dried, 200ul

Validity: 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Gorwth conditions: 28 ℃, aerobic, 5-7 days, comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4*7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 & plusmn;0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

Item	test results
viability:	good viability, in 5-7 days plate colony is obvious
colony morphology: (above)	small filamentous fungi with obvious colonies on comprehensive PDA medium, The initial hyphae are white, dense and vigorous, and yellow spores are produced later.
Conclusion:	good viability, no abnormal colony morphology, qualified

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