BeNa Culture Collection
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| Culture medium | BNCC362337 |
| Description | Chocolate Agar Plate |
| Composition | Polypeptone:18.0g, Starch:1.0g, NaCl:5.0g, Agar:15.0g, Defibrinated Sheep Blood:40mL, pH:7.4±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48h；5%CO2； |
| Subculture procedure | ①Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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