Clostridium sporogenes|247082 |BNCC

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Clostridium sporogenes (Metchnikoff) Bergey et al.

Subculture procedure	① Prepare 2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush with forceps; ③ Aspirate ~0.5mL sterile water (previously equilibrated in anaerobic conditions for 24h) into a lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates at 200μL/Agar plate, spreading evenly; ④ Incubate plates under the specified conditions for 48-72h.
Growth conditions	37°C, 2-3 days, anaerobic
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Irregular Margins: Irregular Transparency: OpaqueColor: White Elevation: Flat Surface: Smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

Clostridium sporogenes

Storage conditions : 2~8 ℃

No. 247082

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, Colombian blood plate (ready-to-use type),48-72h.

Recovery steps:
(1) Prepare 2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 48-72hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 72h strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Irregular Edge: Irregular Transparency: Opacity color: white uplift: flat surface: smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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