Clostridium sporogenes|247082 |BNCC

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Clostridium sporogenes (Metchnikoff) Bergey et al.

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C, 2-3 days, anaerobic
Subculture procedure	① Prepare 2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush with forceps; ③ Aspirate ~0.5mL sterile water (previously equilibrated in anaerobic conditions for 24h) into a lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates at 200μL/Agar plate, spreading evenly; ④ Incubate plates under the specified conditions for 48-72h.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Irregular Margins: Irregular Transparency: OpaqueColor: White Elevation: Flat Surface: Smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing