Aspergillus flavus|336717 |BNCC

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Aspergillus flavus Link

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, initially white hyphae, dense and vigorous, and later producing yellow spores that grow on flat plates
Sharing mode	Public welfare sharing

1.Description

1. Name:Aspergillus flavus Link

2. BNCC No.:336717

3. Biosafety level : 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. PDA: take 200 grams of peeled potatoes, cut them into small pieces, add 1000 milliliters of water to boil for 30 minutes, filter out potato pieces, supplement the filtrate to 1000 milliliters, add 20 grams of glucose and 15 grams of agar, dissolve them, split them, sterilize 15 pounds for 30 minutes.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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