Neisseria gonorrhoeae|242557 |BNCC

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Neisseria gonorrhoeae (Zopf) Trevisan

Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Disinfect the surface of the carrier tablet and open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a bus straw, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃, 3-4 days, 5% CO2+95% air
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), cocci, purity: pure
Sharing mode	Public welfare sharing

Neisseria gonorrhoeae

Storage conditions: 2~8 ℃

No. 242557

Product format: 90mm flat plate

Validity period: growing culture, in 10 days

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,5% CO₂, chocolate plate (ready-to-use type),3-5 days.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Sterilize the plate surface, open it in biosafety cabinet；

(3) Pick up 1-2 rings of plate colonies by inoculation loop, inoculate to fresh medium；

(4) Put the plates under the above culture conditions for cultivation for 3-5 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 4 days the plate layer is thin and the colony is typical
colony morphology: (above)	Size: 0.5-1mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white bulge: middle bulge surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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