Clostridium difficile 545|244976 |BNCC

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Clostridium difficile (Hall and OToole) Prevot

Subculture procedure	① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 48-72h; anaerobic;
Storage conditions	-80 ℃
Safety level	2
morphology	The diameter of the colony is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing

Clostridium difficile

Storage conditions: 2~8 ℃

No. 244976

Product format: 90mm flat plate

Validity period: growing culture, in 10 days

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),48-72h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the flat surface in the safety cabinet after disinfection;

(3) Use a sterile inoculation ring to pick the plate colony 1-2 ring and transfer it to a new medium;

(4) Put the plates under the above culture conditions for cultivation for 48-72 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability：	good viability, in 72h strain layer become obvious, colony is typical
Colony morphology: (pictured above)	size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque ; Color: white uplift: flat; Surface: bright and smooth ; Texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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