Staphylococcus aureus subsp. aureus Rosenbach

Strain number : BNCC247216 

Lot number : 220608

Biosafety  level: 2 , handle in safety cabinet

Provide format: Freeze-dried powder, beveled ,bacterial liquid, plate

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Medium: nutrient gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Add 5 mgMnSO4 & middot when culturing Bacillus; H2O is beneficial to spore production. Culture conditions: 37 ℃;18-24h; Aerobic

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Viability: good viability, in 24h liquid medium become turbid, strain layer is obvious; scribed plate, typical colony

Morphological characteristics : colony diameter is 1-2mm, round, edge neat, opaque, front off-white, back off-white, middle convex, smooth surface, bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), cocci, purity: pure

Quality inspection chart :

Quality inspection conclusion : good viability, no abnormal colony morphology, qualified