Fusarium oxysporum|336354 |BNCC

Essential Information References Certificate Related Products

Fusarium oxysporum

Subculture procedure	① Dissolve the Frozen vial in a water bath (fungi at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Spread and grow on a comprehensive potato culture medium, filamentous with irregular edges, white mycelium on the front, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name:Fusarium oxysporum

2.BNCC No.:336354

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural.

2. Atmosphere:aerobic

3.Temperature: 25-28 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate