Lactococcus lactis NZ9000|359222 |BNCC

Essential Information Related Products

Lactococcus lactis NZ9000

Culture medium	BNCC361331
Description	M17 Medium with 0.5% Glucose
Composition	Soy Peptone:5.0g, Peptone:2.5g, Casein Peptone:2.5g, Yeast Extract Powder:2.5g, Beef Infusion Powder:5.0g, Lactose:5.0g, Glucose:5.0g, C₆H₇NaO₆:0.5g, C₃H₇Na₂O₆P:19.0g, MgSO₄:0.25g, Agar:12.75g, pH:7.2±0.2(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing