Escherichia coli expression bacterium Rosetta (DE3) Strain|341754 |BNCC

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Rosetta(DE3) Strain

Culture medium	BNCC360993
Description	LB Medium with 50μg/mL Chloramphenicol
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, Chloramphenicol:50μg/mL, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony morphology: Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey White Rise Degree: Flat Surface: Smooth Gray Texture: Moist and easy to pick up
Sharing mode	Public welfare sharing