BL21 (DE3) pLysS Escherichia coli|353807 |BNCC

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Escherichia coli BL21(DE3)pLysS

Culture medium	BNCC360993
Description	LB Medium with 50μg/mL Chloramphenicol
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, Chloramphenicol:50μg/mL, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μ L of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, moist texture, easy to pick
Sharing mode	Public welfare sharing