Escherichia coli expression bacterium Rosetta gami (DE3) pLysS|357925 |BNCC

Essential Information Certificate Related Products

Rosetta-gami(DE3)pLysS

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front, smooth and bright on the surface, moist texture, no pigment, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Escherichia coli Expressing Bacteria Rosetta-gami(DE3)

Storage conditions : 2~8 ℃

No. : 357925

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, aerobic, LB agar medium, 24h. LB agar medium: yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, agar 15.0g (not included in liquid medium), distilled water 1.0 L,pH 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare 1 test tube containing 5~10mL liquid medium and 2 plates;

② Open it in the safety cabinet, burn the top with an alcohol lamp, then quickly drip sterile water to break it, and then smash it with tweezers;

③ Pipette 0.5mL of liquid medium into the freeze-drying tube, dissolve it fully and pour it back into the liquid test tube, and mix well;

④ Pipette 0.2mL of bacterial suspension into the plate, spread evenly, repeat twice to obtain two plates;

⑤ Put all the liquid test tubes and plates under the above-mentioned culture conditions, and the bacteria can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability :	good viability， in 24 hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
colony morphology (above)	Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque color: off-white uplift: flat surface: bright and smooth texture: moist and easy to stir
Conclusion	good resurrection, no abnormal colony morphology, qualified

Download certificate