Escherichia coli SHuffle T7 Express lysY|358923 |BNCC

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SHuffle T7 Express lysY

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24-hour aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, and does not produce
Sharing mode	Public welfare sharing

SHuffle T7 Express lysY SHuffle T7 Express lysY

Storage conditions : 2~8 ℃

No. : 358923

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if the goods are overdue, it will be deemed as good. Dry powder shall not be retained after one use, and can be used after activation for 1~2 generations. please refer to the attached page for the use of inclined plane and bacterial liquid. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions::37 ℃, aerobic, nutrient gravy medium, 24h. Nutritional gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare a test tube containing 5~10mL of liquid culture medium and 2 plates;

② Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ suck 0.5mL of liquid culture medium into freeze-dried tube, fully dissolve and return to liquid test tube again, and mix well;

④ Absorb 0.2mL of bacterial suspension into the plate, coat evenly, and repeat twice to obtain two plates;

⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 24 hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex surface: bright and smooth texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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