BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352243 |
| Description | LB nutrient agar |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10mL liquid medium and two agar plates;② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it into pieces using forceps; ③ Pipette 0.5mL of liquid medium into the freeze-dried vial. After complete dissolution, transfer it back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates;⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Circular, irregular edges, opaque, grayish-white on both sides, centrally convex, smooth surface, glossy appearance, moist texture, easily lifted, G− (red), bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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