BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | Cultivation temperature 37 ℃; Cultivation time 24-48 hours; Aerobic gas environment |
| Subculture procedure | The sample needs to be dissolved in a total of 5 mL of sterile water or sterile physiological saline. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biosafety cabinet and immediately add 5 mL of sterile water. After dissolution, shake the penicillin bottle and shake it evenly to obtain a negative quality control sample, totaling 5 mL with a concentration of 300-3000 CFU/mL. The sample needs to be dissolved in a total of 5 mL of sterile water or sterile physiological saline. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biosafety cabinet and immediately add 5 mL of sterile water. Dissolve it and shake the penicillin bottle evenly to obtain a negative quality control sample, totaling 5 mL with a concentration of 1360 CFU/mL. |
| Storage conditions | -20 ℃, stored in the dark |
| Sharing mode | Public welfare sharing |
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