Luminescent Bacteria Quantitative Strain|392561 |BNCC

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Quantitative strains of photobacterium phosphoreum

Culture medium	BNCC353703
Description	2216E agar
Composition	Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC₆H₅O₇):0.1g, KBr:0.08g, NaCl:19.45g, MgCl₂:5.98g, Na₂SO₄:3.24g, CaCl₂:1.8g, KCl:0.55g, Na₂CO₃:0.16g, Na₂NO₃:0.0016g, Na₂HPO₄:0.008g, SrCl₂:0.034g, H₃BO₃:0.022g, Na₂SiO₃:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃)
Growth conditions	20°C; 24-48h; Aerobic
Subculture procedure	① Cultivate bacteria into suspension according to culture conditions;② Measure the absorbance of the bacterial suspension and calculate its initial concentration using the standard formula; ③ Multiply the initial concentration by the viability rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension to the target concentration based on the actual lyophilized concentration, then aliquot and freeze-dry;
Storage conditions	-20°C
morphology	Colony diameter: 1-2mm, circular, with neat edges; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; easily lifted; G- (red); bacillus; purity: pure
Sharing mode	Public welfare sharing