Quantitative Strain of Escherichia coli|354945 |BNCC

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Quantitative strains of escherichia coli (Migula) Castellani and Chalmers

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; Aerobic;
Subculture procedure	① Cultivate bacteria into suspension according to culture conditions; ② Measure suspension absorbance and calculate initial concentration using standard formula; ③ Multiply initial concentration by survival rate to obtain actual lyophilized concentration;④ Dilute the bacterial suspension to target concentration based on the actual post-lyophilization concentration, then aliquot and freeze-dry;
Storage conditions	-20°C
morphology	Colony diameter 1-2mm, circular, with neat edges, opaque, grayish-white upper surface, raised center, smooth surface, glossy appearance, moist texture, easily lifted, G− (red), bacillus
Sharing mode	Public welfare sharing