BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352252 |
| Description | Brain Heart Infusion Agar |
| Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25℃) |
| Growth conditions | 37°C; 18-24h, aerobic; |
| Subculture procedure | ① First cultivate bacteria into a bacterial suspension under specified conditions; ② Measure the absorbance of the bacterial suspension and calculate its initial concentration using the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension to the target concentration based on the actual post-lyophilization concentration, then aliquot and lyophilize; |
| Storage conditions | -20°C |
| morphology | Colony diameter: 1-2mm, circular, with neat edges; opaque, grayish-white on both sides; centrally raised, smooth surface, glossy appearance, moist texture, easily lifted; G+ (blue-violet), cocci; Purity: Pure |
| Sharing mode | Public welfare sharing |
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