BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; Aerobic |
| Subculture procedure | ① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it; |
| Storage conditions | -20 ℃ |
| morphology | The texture is fluffy, with black spores produced in the later stage. The reverse side is light yellow brown with radial grooves. According to the arrow indication on the penicillin bottle cap, open the strain and dissolve the solution; ② Pour the solution into the bacterial slide, cover it with a rubber stopper, and shake it to dissolve; ③ After sufficient dissolution, extract according to the experimental requirements in proportion; ④ Please use it immediately after opening and do not store it; |
| Sharing mode | Public welfare sharing |
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