Quantitative strain of Bacillus subtilis|388990 |BNCC

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Quantitative strains of bacillus subtilis subsp. spizizenii Nakamura et al.

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, bright on the surface, moist in texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus, purity: Pure
Sharing mode	Public welfare sharing