Quantitative strain of Bacillus subtilis|392940 |BNCC

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Quantitative strains of bacillus subtilis subsp. spizizenii Nakamura et al.

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	1. Open the bacterial strain and dissolution solution according to the arrow indication on the penicillin bottle cap. 2. Take 1mL of the solution and add it to the quantitative bacterial slide. Cover with a rubber stopper and shake to dissolve. 3. After sufficient dissolution, extract according to the experimental requirements in proportion.
Storage conditions	-20 ℃
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, white on the front, raised in the middle, rough on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing