Quantitative strain of Listeria monocytogenes|393538 |BNCC

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Quantitative strains of listeria ivanovii

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front, raised in the middle, smooth on the surface, bright on the surface, moist in texture, does not produce pigments, G+(blue purple), Bacillus
Sharing mode	Public welfare sharing