Bacillus subtilis spore counting standard substance|359088 |BNCC

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Reference material of bacillus subtilis spores count

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃, aerobic, 18-24h
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃, dark storage
morphology	The colony diameter is 2-4mm, circular, with wavy edges, opaque, gray white on the front, flat in shape, rough on the surface, dry in texture, G+(blue purple), Bacillus
Sharing mode	Public welfare sharing