Quantitative strain of Streptococcus agalactiae|340014 |BNCC

Essential Information Related Products

Quantitative strains of streptococcus agalactiae

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 18-24h; 5% CO2
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, white on the front, light in color, raised in the middle, smooth on the surface, bright on the surface, moist in texture, easy to pick up, does not produce pigments, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing