Quantitative strain of Vibrio parahaemolyticus|362667 |BNCC

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Quantitative strains of vibrio parahaemolyticus

Culture medium	BNCC353940
Description	3.5% Sodium Chloride Agar Medium
Composition	Beef Extract:3.0g, Peptone:10.0g, NaCl:35.0g, Agar:15.0g, pH:7.2-7.4(25℃)
Growth conditions	30 ℃; 18-24h; aerobic;
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃
morphology	The colony diameter is 1-2mm, circular, with irregular edges, semi transparent, gray white on the front, protruding in the middle, bright and smooth on the surface, moist texture, easy to pick up, G - (red), purity: pure
Sharing mode	Public welfare sharing