Quantitative strain of Staphylococcus aureus|381462 |BNCC

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Quantitative strains of staphylococcus aureus

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; Aerobic
Subculture procedure	① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it;
Storage conditions	-20 ℃
morphology	Round, with neat edges, opaque, golden yellow on the front, light colored, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, non pigmented, G+(blue purple), cocci
Sharing mode	Public welfare sharing