BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352252 |
| Description | Brain Heart Infusion Agar |
| Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; aerobic; |
| Subculture procedure | ① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it; |
| Storage conditions | -20 ℃ |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, orange on the front, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis |
| Sharing mode | Public welfare sharing |
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