BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | Host culture: 37 ℃, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 20.0g agar (excluding liquid medium), 1.0L distilled water, pH 7.0. Sterilize at 121 ℃ for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake at 37 ℃, 160-180 rpm until OD600 is 0.1-0.4; ② Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ④ Cultivation: Place the triangular flask at 37 ℃ and incubate overnight on a shaker at 160-180rpm; ⑤ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes, filter the lysate using a 0.22 μ m sterile filter, and store the filtrate at 4 ℃ |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
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