BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC363070 |
| Description | Tryptic Soy Agar (TSA) |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic |
| Subculture procedure | phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL tryptone soy broth culture medium, shake at 30 ℃, 160-180 rpm until OD600 is 0.4-0.6; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 30 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 30 ℃ and shake at 160-180rpm for 48 hours; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it with a 0.22 μ m sterile filter, and collect the filtrate. The filtrate can be stored at 4 ℃ |
| Storage conditions | -80 ℃ |
| Sharing mode | Public welfare sharing |
Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method))
BNCC372790
Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method)(Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method))
BNCC373716
Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method))
BNCC375969
Pig origin nucleic acid detection kit (Fluorescent PCR method)(Pig origin nucleic acid detection kit (Fluorescent PCR method))
BNCC378053
Biochemical identification kit for Corynebacterium kutscheri(Biochemical identification kit for Corynebacterium kutscheri)
BNCC378878
Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method))
BNCC381624
rimers for the detection of Escherichia coli
BNCC393979
Candida haemulonii DNA lab quality control
BNCC393760
Genomic DNA from Pseudomonas aeruginosa
BNCC393719
pACYC-MS2-EBOV-NP
BNCC393711
Human adenovirus 3 antigen test reference (Chemically inactivated) (Strongly positive)
BNCC393602
pDuRCC-K
BNCC393599
- English
- Japanese