BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352243 |
| Description | LB nutrient agar |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic; |
| Subculture procedure | ① Host bacterial preparation: Pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake and culture at 37 ℃, 160-180 rpm until OD600 is 0.1-0.4; ② Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ④ Cultivation: Place the triangular flask at 37 ℃ and incubate overnight on a shaker at 160-180rpm; ⑤ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes. Filter the lysate using a 0.2 μ m sterile filter, which can be filtered twice to completely remove the host. The filtrate can be stored at 4 ℃ |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method))
BNCC372790
Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method)(Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method))
BNCC373716
Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method))
BNCC375969
Pig origin nucleic acid detection kit (Fluorescent PCR method)(Pig origin nucleic acid detection kit (Fluorescent PCR method))
BNCC378053
Biochemical identification kit for Corynebacterium kutscheri(Biochemical identification kit for Corynebacterium kutscheri)
BNCC378878
Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method))
BNCC381624
Pseudomonas sp DNA lab quality control
BNCC394019
Human herpesvirus 1 DNA lab quality control
BNCC393892
Candida duobushaemulonii DNA standard
BNCC393694
Puumala virus pseudovirus ribonucleic acid liquid indoor quality control product
BNCC393634
Measles virus RNA standard
BNCC393629
Barnettozyma californica
BNCC393610
- English
- Japanese