BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | Phage culture: ① Host bacterial preparation: Pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth culture medium, shake and culture at 37 ℃, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 37 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 ℃ and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and collect the filtrate through a 0.22 μ m sterile filter. The filtrate can be stored at 4 ℃ |
| Storage conditions | -80 ℃ |
| Sharing mode | Public welfare sharing |
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