BeNa Culture Collection
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| Culture medium | BNCC369667 |
| Description | Nutrient Broth (NB) |
| Composition | Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | Nutrient broth medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 1.0L distilled water, pH 7.0. Sterilize at 121 ℃ for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake at 37 ℃, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 37 ℃ water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 ℃ and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and collect the filtrate through a 0.22 μ m sterile filter. The filtrate can be stored at 4 ℃ |
| Storage conditions | -80 ℃ |
| Sharing mode | Public welfare sharing |
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