BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic; |
| Subculture procedure | Host culture: 37 ℃, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 20.0g agar (excluding liquid medium), 1.0L distilled water, pH 7.0. Sterilize at 121 ℃ for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50 mL nutrient broth medium, shake at 37 ℃, 160-180 rpm until OD600 is 0.1-0.4; ② Transfer: Transfer an appropriate amount of host bacterial solution into the Agar plate, wait for the host bacterial solution to dry, and then apply bacteriophages onto the Agar plate; ③ Cultivation: Place the petri dish in a 37 ℃ incubator overnight for cultivation; ④ Bacterial culture: After 24 hours, plaques grow on the agar plate. Wash the agar plate and bacteriophages completely into a 40mL triangular flask and incubate overnight at 37 ℃ and 160-180rpm on a shaker. ⑤ Filtration: After 16-18 hours, centrifuge the bacteriophage culture at 4000g for 10 minutes and filter the lysate using a 0.22 μ m sterile filter. The filtrate can be stored at 4 ℃ |
| Storage conditions | Liquid nitrogen |
| Sharing mode | Public welfare sharing |
Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method))
BNCC372790
Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method)(Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method))
BNCC373716
Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method))
BNCC375969
Pig origin nucleic acid detection kit (Fluorescent PCR method)(Pig origin nucleic acid detection kit (Fluorescent PCR method))
BNCC378053
Biochemical identification kit for Corynebacterium kutscheri(Biochemical identification kit for Corynebacterium kutscheri)
BNCC378878
Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 21 nucleic acid detection kit (Fluorescent PCR method))
BNCC381624
Mycobacterium tuberculosis H37Ra lab quality control
BNCC393904
Neisseria gonorrhoeae DNA lab quality control
BNCC393896
Candida haemulonii DNA standard
BNCC393744
Human adenovirus 18 DNA standard
BNCC393736
Haemophilus influenzae DNA lab quality control
BNCC393619
Hanseniaspora uvarum
BNCC393611
- English
- Japanese