BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC363644 |
| Description | Fastidious Anaerobe Broth (FAB) |
| Composition | Mixed Peptone:15.0g, Yeast Extract Powder:10.0g, Sodium Thioglycolate(C2H3NaO2S):0.5g, NaCl:2.5g, Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, Hemin:0.005g, Vitamin K:0.0005g, NaHCO3:0.4g, Resazurin:0.001g, Agar:0.75g, pH:7.2±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | liquid reproduction in a selective anaerobic broth (FAB). Inoculate the overnight culture of P. distason into fresh FAB medium at a ratio of 1:20 and anaerobically culture at 37 ° C. When the culture is at OD600nm~0.3, inoculate with φ PDS1 and anaerobically culture overnight at 37 ° C. PDS1 can be stored at 4 ° C and stored in FAB medium and 50% glycerol at -80 ° C |
| Storage conditions | Liquid nitrogen |
| Sharing mode | Public welfare sharing |
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