BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361988 |
| Description | EMEM Complete Medium (with 2% FBS) |
| Composition | 98% EMEM+2% FBS |
| Growth conditions | 35°C; 5% CO₂ + 95% air |
| Subculture procedure | (1) Parasite inoculation: Remove Trichomonas vaginalis from -80°C freezer and rapidly thaw in a 37°C water bath. Inoculate 500μL of undiluted parasite suspension per tube and incubate at 35°C under anaerobic conditions for 2-3 days. (2) Parasite Collection: Begin collection after 3 days of parasite culture. Harvest by centrifuging at 1500 rpm for 10 min, collect the pellet, and store at -80°C. |
| Storage conditions | Liquid nitrogen |
| Sharing mode | Public welfare sharing |
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