Trichomonas oralis|390631 |BNCC

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Pentatrichomonas hominis

Growth conditions	25-37 ° C
Subculture procedure	When the culture medium reaches or approaches peak density, ice the test tube for 10 minutes. Gently invert the culture tube 10 times and transfer sterile 0.1 mL to 0.4 mL of the aliquots to a covered test tube containing 13 mL of ATCC medium 2154. The culture is tilted horizontally at 15 ° C at 35 ° C. Transfer the culture medium every 3 to 4 days, as described in steps 1 to 2. The transfer interval depends on the quantity of inoculum and the quality of the culture medium. This should be determined through daily empirical cultural checks until the growth cycle stabilizes. Do not allow culture to overgrow
Storage conditions	Liquid nitrogen
Sharing mode	Public welfare sharing