BeNa Culture Collection
info@bncc.com
| Growth conditions | 37°C; 5% CO₂ + 95% air; |
| Subculture procedure | ① Inoculation procedure: Seed host cells in T25 flasks 24-48 hours prior to inoculation. Prepare for viral inoculation when host cell density reaches 80-90%; Remove cell culture medium and inoculate with 100–500 μL of viral suspension. Allow adsorption for 1–2 hours under culture conditions, gently swirling the T25 flask every 15–30 minutes to ensure even viral distribution. Add 6 mL of viral growth medium to complete adsorption, then transfer to the incubator for continued culture. Monitor for cytopathic effects periodically. ② Virus Harvesting: Initiate virus collection once host cells exhibit complete cytopathic changes. During collection, freeze-thaw cells 1–2 times, then filter through a 0.22μm pore size filter. The collected filtrate constitutes the virus solution. |
| Storage conditions | Liquid nitrogen |
| Sharing mode | Public welfare sharing |
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