BeNa Culture Collection
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| Growth conditions | 37 ℃; 5% CO2+95% air; |
| Subculture procedure | ① Remove the cryovial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the cryovial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the suspension to 6-7 mL of complete culture medium and incubate in an incubator. ③ Pay attention to changes in the pH value of the culture medium and cell density. When the cell density reaches greater than 2 × 106 cells/mL, repeat the passaging operation or cryopreservation. |
| Storage conditions | liquid nitrogen |
| Sharing mode | Public welfare sharing |
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