BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361988 |
| Description | EMEM Complete Medium (with 2% FBS) |
| Composition | 98% EMEM+2% FBS |
| Growth conditions | 35-37 ℃; 5% CO2+95% air; |
| Subculture procedure | ① Remove cryovials from liquid nitrogen or -80°C freezer, place in PE gloves, and quickly immerse in 37°C water bath. Shake cryovials to accelerate thawing; aim for complete thawing within 1 minute. ② Add the suspension to 6-7 mL of complete medium and incubate in a culture incubator. ③ Monitor medium pH and cell density. When cell density exceeds 2 × 10⁶ cells/mL, perform passage or cryopreservation. |
| Storage conditions | liquid nitrogen |
| Sharing mode | Public welfare sharing |
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