BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC391550 |
| Description | Ureaplasma Type 2 Medium |
| Composition | PPLO Broth + 0.4% Bromothymol Blue Ethanol Solution:1mL + 10% Inactivated Horse Serum + Glycyl-L-Histidyl-L-Lysine 2µg + 10% Urea 0.25mL. |
| Growth conditions | 37°C; 2-3 days; Microaerophilic (3%-5% O₂, 10% CO₂); |
| Subculture procedure | Remove one vial of frozen ureaplasma stock (500 μL) and six tubes containing prepared medium (one as a control tube; 5 for culture). Add all thawed ureaplasma to the first tube (containing 4.5mL prepared ureaplasma medium), mix thoroughly, then transfer 0.5mL to the next culture tube. Repeat to create 5 dilution gradients. Incubate all 5 diluted tubes and 1 control tube aerobically at 37°C for 18 hours. |
| Storage conditions | liquid nitrogen |
| Sharing mode | Public welfare sharing |
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